Transcription profiling of mouse tet inducible NIH 3T3 cells expressing ATPase-defective BRG-1 to identify SWI-SNF-Regulated Genes

ABSTRACT: NIH 3T3 cells (mouse fibroblasts) were engineered such that the dominant negative ATPase-defective BRG-1 is expressed via the tet-off inducible expression system to inactivate the SWI-SNF chromatin remodeling complexes (Mol Cell Biol 20, 2839). These cell lines have been used for several studies to demonstrate the in vivo functions of the SWI-SNF complexes in transcription, differentiation and cell cycle control. We used one of those cell line designated as B05-1. B05-1 cells were cultured in the medium with tetracycline for routine maintenance, and cultured in the medium without tetracycline for four days to maximally induce the expression of the dominant negative BRG-1, which we designated as B05-1(+tet) and B05-1(-tet) cells, respectively. The aim of our microarray experiments was to de

INSTRUMENT(S): GenePix 4200A

ORGANISM(S): Mus musculus

SUBMITTER: Anthony Imbalzano 

PROVIDER: E-MEXP-434 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress