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Platform and RNA amplification method comparisons using Universal Human Reference RNA and Caki-1 RNA on Agilent Human 1A Oligo microarrays and Affymetrix HG-U133Plus 2.0 GeneChips

ABSTRACT: The profiles of gene transcripts from 3 indipendent samples of Universal Human Reference RNA and Caki-1 RNA, amplified into biotinylated cRNA according to 1) standard Affymetrix procedure 'one-cycle target labeling' 2) standard Affymetrix procedure 'two cycle target labeling' 3) Transplex WTA method, were hybridized on HG-U133Plus 2.0 GeneChips and compared. In addition we performed the same gene expression comparison using not amplified HHR and Caki1 RNA samples on Agilent Human 1A Oligo microarrays.

INSTRUMENT(S): G2565BA DNA microarray scanner [Agilent]

ORGANISM(S): Homo sapiens

DISEASE(S): renal cell carcinoma

SUBMITTER: federica viti

PROVIDER: E-MEXP-448 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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