Platform comparison and transcription profiling of human cell lines on three array platforms (Affymetrix GeneChip Human Genome U133 Plus 2.0, GE Healthcare/Amersham Biosciences UniSet Human 20K I Bioarray and Agilent Whole Human Genome Oligo Microarray)

ABSTRACT: A "Cartes d'Identite des Tumeurs" (CIT) project from the french Ligue Nationale Contre le Cancer (http://cit.ligue-cancer.net). We compared the relative precision and accuracy of expression measurements obtained from three different state-of-the-art commercial short and long-oligonucleotide microarray platforms (Affymetrix GeneChipTM, GE Healthcare CodeLinkTM and Agilent Technologies). The design of the comparison was chosen to judge each platform in the context of a multi-project program. The analyses were based on data from three biologically distinct human cell lines and a non-redundant set of roughly 3,400 targets chosen for their unique correspondence toward a single transcript. Raw data from the following were studied : 12 Affymetrix HG-U133 plus 2.0 GeneChipTM arrays (3

INSTRUMENT(S): G2565BA DNA microarray scanner [Agilent], GenePix 4000B [Axon]

ORGANISM(S): Homo sapiens

DISEASE(S): breast cancer

SUBMITTER: Fabien Petel 

PROVIDER: E-MEXP-467 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress