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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of wild type and rpd3- yeast cells in response to osmotic stress

ABSTRACT: Comparison of wild type and rpd3- S. cerevisiae cells in response to osmotic stress. Wild type (MATalpha ura3 leu2 his3) and rpd3- (MATalpha ura3 leu2 his3 rpd3::KANMX) were grown to OD660=1 in YPD media and then subjected or not to a brief osmotic stress (5 or 20 min / 0.4 M NaCl). Cells were collected by centrifugation and total RNA was extracted as described.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Glòria Mas

PROVIDER: E-MEXP-48 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The MAPK Hog1 recruits Rpd3 histone deacetylase to activate osmoresponsive genes.

De Nadal Eulàlia E Zapater Meritxell M Alepuz Paula M PM Sumoy Lauro L Mas Glòria G Posas Francesc F

Nature 20040101 6972

Regulation of gene expression by mitogen-activated protein kinases (MAPKs) is essential for proper cell adaptation to extracellular stimuli. Exposure of yeast cells to high osmolarity results in rapid activation of the MAPK Hog1, which coordinates the transcriptional programme required for cell survival on osmostress. The mechanisms by which Hog1 and MAPKs in general regulate gene expression are not completely understood, although Hog1 can modify some transcription factors. Here we propose that ...[more]

PMID: 14737171

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