Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of ethylene-dependent responses to short day exposure in birch apices


ABSTRACT: We have used transgenic ethylene-insensitive birches (Betula pendula), which express the Arabidopsis ethylene receptor gene ETR1 carrying the dominant mutation etr1-1, to investigate the role of ethylene in short day (SD) -induced responses in the shoot apical meristem in birch. Wild-type birch (clone V5834) and two ethylene-insensitive lines in this background (BPetr1-1-35 and BPetr1-1-86; see Plant Physiol 132: 185-195) were exposed to SD. After 12, 16 and 20 days under SD, apices of branches of three trees were pooled before RNA extraction from each sample. To study the ethylene-dependent SD-transcriptome in birch apices, the RNA extracts of lines BPetr1-1-35 and BPetr1-1-86 were separately compared with the reference, wild-type V5834, at the three time points (12SD, 16SD, 20SD) resulting in altogether six microarray hybridizations.

INSTRUMENT(S): ScanArray 5000 [PerkinElmer]

ORGANISM(S): Betula pendula

SUBMITTER: Raili Ruonala 

PROVIDER: E-MEXP-491 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transitions in the functioning of the shoot apical meristem in birch (Betula pendula) involve ethylene.

Ruonala Raili R   Rinne Päivi L H PL   Baghour Mourad M   Moritz Thomas T   Tuominen Hannele H   Kangasjärvi Jaakko J  

The Plant journal : for cell and molecular biology 20060501 4


In many trees, a short photoperiod (SD) triggers substantial physiological adjustments necessary for over-wintering. We have used transgenic ethylene-insensitive birches (Betula pendula), which express the Arabidopsis ethylene receptor gene ETR1 carrying the dominant mutation etr1-1, to investigate the role of ethylene in SD-induced responses in the shoot apical meristem (SAM). Under SD, the ethylene-insensitive trees ceased elongation growth comparably to the wild-type. In contrast, the formati  ...[more]

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