Transcription profiling of ethylene-dependent responses to short day exposure in birch apices

ABSTRACT: We have used transgenic ethylene-insensitive birches (Betula pendula), which express the Arabidopsis ethylene receptor gene ETR1 carrying the dominant mutation etr1-1, to investigate the role of ethylene in short day (SD) -induced responses in the shoot apical meristem in birch. Wild-type birch (clone V5834) and two ethylene-insensitive lines in this background (BPetr1-1-35 and BPetr1-1-86; see Plant Physiol 132: 185-195) were exposed to SD. After 12, 16 and 20 days under SD, apices of branches of three trees were pooled before RNA extraction from each sample. To study the ethylene-dependent SD-transcriptome in birch apices, the RNA extracts of lines BPetr1-1-35 and BPetr1-1-86 were separately compared with the reference, wild-type V5834, at the three time points (12SD, 16SD, 20SD) resulti

INSTRUMENT(S): ScanArray 5000 [PerkinElmer]

ORGANISM(S): Betula pendula

SUBMITTER: Raili Ruonala 

PROVIDER: E-MEXP-491 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress