Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse embryonic stem cells from cloned and fertilized blastocysts

ABSTRACT: Comparison of transcriptional profiles from five ES cell lines derived by nuclear transplantation from B and T cells or from fibroblasts and transcriptional profiles from five fertilization-derived ES cell lines of matching genetic backgrounds.

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Mus musculus

SUBMITTER: Tobias Brambrink

PROVIDER: E-MEXP-501 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

ES cells derived from cloned and fertilized blastocysts are transcriptionally and functionally indistinguishable.

Brambrink Tobias T Hochedlinger Konrad K Bell George G Jaenisch Rudolf R

Proceedings of the National Academy of Sciences of the United States of America 20060117 4

Reproductive cloning is uniformly rejected as a valid technology in humans because of the severely abnormal phenotypes seen in cloned animals. Gene expression aberrations observed in tissues of cloned animals have also raised concerns regarding the therapeutic application of "customized" embryonic stem (ES) cells derived by nuclear transplantation (NT) from a patient's somatic cells. Although previous experiments in mice have demonstrated that the developmental potential of ES cells derived from ...[more]

PMID: 16418286

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Project description:Primary murine fetal liver cells were freshly isolated from day e14.5 livers and then sorted for successive differentiation stages by Ter119 and CD71 surface expression (ranging from double-negative CFU-Es to Ter-119 positive enucleated erythrocytes) [Zhang, et al. Blood. 2003 Dec 1; 102(12):3938-46]. RNA isolated from each freshly isolated, stage-sorted population was reverse-transcribed, labelled, and then hybridized onto 3' oligo Affymetrix arrays. Important erythroid specific genes as well as the proteins that regulate them were elucidated through this profiling based on coexpression and differential expression patterns as well as by extracting specific GO categories of genes (such as DNA-binding proteins). Abstract (submitted paper): rationale for expression profiling Gene-targeting experiments report that the homeodomain-interacting protein kinases 1 and 2, Hipk1 and Hipk2, are essential but redundant in hematopoietic developmentâbecause Hipk1/Hipk2 double-deficient animals exhibit severe defects in hematopoiesis and vasculogenesis while the single knockouts do not. These serine-threonine kinases phosphorylate, and consequently modify the functions of, several important hematopoietic transcription factors and cofactors. Here we show that Hipk2 knockdown alone plays a significant role in terminal fetal liver erythroid differentiation. Hipk1 and Hipk2 are highly induced during primary mouse fetal liver erythropoiesis. Specific knockdown of Hipk2 inhibits terminal erythroid cell proliferationâexplained in part by impaired cell cycle progression as well as increased apoptosisâand terminal enucleation as well as the accumulation of hemoglobin. Hipk2 knockdown also reduces the transcription of many genes involved in proliferation and apoptosis as well as important, erythroid-specific genes involved in hemoglobin biosynthesisâsuch as alpha-globin and mitoferrin 1âdemonstrating that Hipk2 plays an important role in some but not all aspects of normal terminal erythroid differentiation.

Dataset Information

Transcription profiling of mouse embryonic stem cells from cloned and fertilized blastocysts

Publications

ES cells derived from cloned and fertilized blastocysts are transcriptionally and functionally indistinguishable.

Similar Datasets

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