Project description:Microarray analyses were performed on Arabidopsis thaliana plants over-expressing either AtERF5 or AtERF6, which are both transcription factors of the ethylene response factor (ERF) family. In this way it was hoped that any changes in the Arabidopsis transcriptome caused by their over-expression would provide clues as to the role(s) these genes play in vivo.<br>Arabidopsis thaliana plants were transformed with either 35S::AtERF5, 35S::AtERF6 or the empty vector pK2GW7 (used as a control). Total RNA was extracted from untreated 10-day old seedlings. Three separate arrays were performed for each of the two over-expressors, (the RNA for each slide having been extracted from an independent biological replicate).
Project description:Microarray analyses were performed on Arabidopsis thaliana plants overexpressing ERF5. In this way it was hoped that any changes in the Arabidopsis transcriptome caused by overexpression would provide clues as to the role this gene plays in vivo.<br>Arabidopsis thaliana plants were transformed with 35S::ERF5 or the empty vector pK2GW7 (used as a control). Total RNA was extracted from untreated 10-day old seedlings. Three separate arrays were performed (the RNA for each slide having been extracted from an independent biological replicate).
Project description:The role of heterotrimeric G protein alpha and beta subunits on methyl jasmonates regualted gene expression using alpha and beta subunit single and double mutants.
Project description:Transcription profiling of fission yeast /yox1/ deletion and genome wide location analysis of Yox1p and Cdc10p transcription factors reveal a negative feedback interaction: /yox1 /is transcriptionally activated by MBF, and Yox1p in turn transcriptionally represses the MBF target genes (including /yox1 /itself).
Project description:Cell cycle timecourse of fission yeast using both selective (elutriation) and whole-culture (temperature-sensitive mutant cdc25) synchronisation methods