Transcription profiling of Mycosphaerella graminicola in different nutritional environments

ABSTRACT: In order to gain a first insight into the Mycosphaerella graminicola global transcriptome in different nutritional environments, we performed initial experiments on two in vitro growth conditions during log-phase growth and on infected plant material twenty-eight days after inoculation. In vitro log phase growth in nutrient-rich Potato Dextrose Broth (PDB) was used as a control in independent comparisons with 1) log phase growth in nutrient-limiting Czapek-Dox Broth (CDB) and 2) twenty-eight days of plant infection. Growth in PDB results in a rapid “budding” type growth of the M. graminicola sporidia. Growth in CDB is phenotypically similar in that the fungus continues to grow as budding sporidia but this occurs at approximately 20% of the rate in PDB. In contrast, late stage infected pla

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Mycosphaerella graminicola

SUBMITTER: John Hargreaves 

PROVIDER: E-MEXP-522 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress