Transcription profiling and translation status analysis in yeast with hydrogen peroxide and cycloheximide treatment

ABSTRACT: Global restriction of protein synthesis is a hallmark of cellular stress. Using hydrogen peroxide, we monitor the transcript level and also the translation status for each RNA using cycloheximide to freeze elongating ribosomes. Polyribosome fractionation of cell extracts was used to separate highly translated and poorly translated mRNAs that were then separately analysed.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Ioulia Smirnova 

PROVIDER: E-MEXP-526 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress