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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of four mouse bone marrow hematopoietic stem cells (HSC) early B lineage stages(MLP, CLP, Fr.A, Fr.B) to identify coordinately regulated gene as cells progress down the B cell development pathway

ABSTRACT: Differentiation from hematopoietic stem cells (HSC) to early B-lineage cells proceeds through a series of intermediate stages, during which cells are thought to become progressively more restricted in their developmental potential. RNAs were prepared from four early B lineage stages(MLP, CLP, Fr.A, Fr.B) from mouse bone marrow, and microarrays were done to identify sets of genes that were coordinately regulated as cells progressed down the B cell development pathway.

INSTRUMENT(S): G2565BA DNA microarray scanner [Agilent]

ORGANISM(S): Mus musculus

SUBMITTER: Susan Shinton

PROVIDER: E-MEXP-559 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Lineage specification and plasticity in CD19- early B cell precursors.

Rumfelt Lynn L LL Zhou Yan Y Rowley Benjamin M BM Shinton Susan A SA Hardy Richard R RR

The Journal of experimental medicine 20060227 3

We describe here three CD19- B cell precursor populations in mouse bone marrow identified using 12-color flow cytometry. Cell transfer experiments indicate lineage potentials consistent with multilineage progenitor (MLP), common lymphoid progenitor (CLP), and B lineage-restricted pre-pro-B (Fr. A), respectively. However, single cell in vitro assays reveal lineage plasticity: lymphoid/myeloid lineage potential for CLP and B/T lineage potential for Fr. A. Despite myeloid potential, recombination a ...[more]

PMID: 16505143

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