Transcription profiling of primary human tubular epithelial cells (HUTEC) response to TGFbeta at three concentrations to study the epithelial to mesenchymal transition

ABSTRACT: This experiment was aimed to study the Epithelial to mesenchimal transition (EMT) induced by TGFbeta in primary tubular epithelial cells. Primary cultures were grown for 4 days using three different TGFbeta1 concentrations: 5 ng/ml, 10 ng/ml and 50 ng/ml. Cells were grown in plastic flasks (Falcon) or in plastic flasks (Falcon) covered by type I collagen. Differentially expressed genes were identified comparing treated vs. control cells maintained for 4 days in 1% serum without TGFbeta1.

INSTRUMENT(S): ScanArray Lite [PerkinElmer]

ORGANISM(S): Homo sapiens

SUBMITTER: Stefano Campanaro 

PROVIDER: E-MEXP-566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress