Transcription profiling of spermatogenesis in juvenile mouse testis and analysis of four germ cell deficient mouse models, time series

ABSTRACT: Temporal study of the first wave of spermatogenesis in juvenile mouse testis and analysis of four germ cell deficient mouse models.
A series of purified testis cell-types (McCarrey libraries)constituting a focussed gene set was exploited to examine gene expression in prepubertal mouse testis. Testis RNA from C57/BL6J mice at various ages post partum was compared to a common control consisting of adult (8 week) testis RNA from the same strain(also corresponding to the last time-point).This generates a time course showing the activation of genes on the array across the first wave of spermatogenesis, which can then be correlated with the appearance of specific germ cell types, allowing assignation of unknown genes to differing cell types.

Adult testis RNA from four different ge

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Mus musculus

SUBMITTER: Peter Ellis 

PROVIDER: E-MEXP-571 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress