Transcription profiling of S. cerevisiae response to nitrosative stress

ABSTRACT: Nitric oxide and NO-derived species (RNS) are defense molecules with broad antimicrobial activity. Micro-organisms have developed strategies to sense RNS and counteract their damaging effects. We used Saccharomyces cerevisiae, harbouring a deletion of YHB1 that encodes the main NO scavenger enzyme, to study consequences of RNS exposure on whole genome transcriptional response. The expression of >700 genes was altered on RNS treatment. No major role for ROS-scavenging enzymes was found, and the respiratory chain, the main site of ROS production, had only minor involvement in the RNS-induced stress. The changes were generally transient and also found after treatment with the respiratory inhibitor myxothiazol. 117 genes however showed a persistent response which was not observed after myxothi

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: BRIGITTE MEUNIER 

PROVIDER: E-MEXP-580 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress