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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse primary dermal fibroblasts treated with DNA demethylating agent 5-aza-2-deoxycytidine

ABSTRACT: Mouse primary dermal fibroblasts were treated with DNA demethylating agent 5-aza-2'-deoxycytidine. The exposure was 5um 5A2dC for 96 hours followed by recovery in normal medium for 24 hours. Three biological replicates were created for both the control and treated populations. Each biological replicate represents a separate derivation of dermal fibroblasts from genetically identical mouse pups aged 0-3 days. Cells reached passage 4 before initiating drug exposure.

ORGANISM(S): Mus musculus

SUBMITTER: Tammy Vallender

PROVIDER: E-MEXP-701 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Localized methylation in the key regulator gene endothelin-1 is associated with cell type-specific transcriptional silencing.

Vallender Tammy W TW Lahn Bruce T BT

FEBS letters 20060714 18

DNA methylation can contribute to the stable transcriptional silencing of mammalian genes. Often times, these genes are important developmental regulators, and their silencing in cell types where they are not supposed to be active is important for the phenotypic stability of the cells. To identify key developmental regulator genes whose expression in terminally differentiated cells may be inhibited by DNA methylation, mouse dermal fibroblasts were demethylated with 5-aza-2'-deoxycytidine, and ch ...[more]

PMID: 16870175

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