Transcription profiling of upper beak primorida of five species of Darwins Finches to identify species and/or size specific transcripts

ABSTRACT: We used five different species of Darwin’s Finches (G. magnirostris, G. conirostris, G. fortis, G. scandens, G. difficilis). G. difficilis was used as a common reference and we polled RNA from 9 unrelated individuals of this species to lower variation. We used at least two unrelated individual embryos from each of the more derived species. We first made cDNA library from the upper beak primordia tissue, which was used to print microarrays. Next, we collected upper beak primordia from stage 26 embryos of 5 species of Darwin’s Finches that differed in their beak morphology, for making Cy3- and Cy5-labeled probes with the most basal species, the Sharp-beaked Finch, serving as a reference. We then clustered the obtained expression profiles to find transcripts that were expressed in a specie

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Geospiza magnirostris

SUBMITTER: Clifford Tabin 

PROVIDER: E-MEXP-702 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress