Transcription profiling of light and carbon signaling interactions in Arabidopsis

ABSTRACT: A. thaliana seeds of Columbia (Col-0) ecotype were surface-sterilized, plated on designated media and vernalized for 48 hours at 8C. Plants were grown semi-hydroponically under 16-hr-light, 8-hr-dark cycles at a constant temperature of 23C on basal Murashige and Skoog medium supplemented with 2 mM KNO3, 2 mM NH4NO3 and 30 mM sucrose. Two-week-old seedlings were transferred to fresh MS media without nitrogen and sucrose and dark-adapted for 48 hours. To perform specific metabolic treatments manipulating availability of source of carbon (C) and illumination (L): -C-L, +C-L, -C+L, +C+L, two-week-old seedlings were transferred to fresh MS medium containing 0% or 1% sucrose and either placed back into the dark or illuminated with white light for an additional 8 hours. The various treatments wer

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Michael Shin 

PROVIDER: E-MEXP-71 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress