Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of stationary phase E.coli wild type and crl::cat mutant strains to identify Crl-regulated genes

ABSTRACT: We wished to identify Crl-regulated genes in stationary phase in E.coli, and whether those overlap with the previously identified regulon of RpoS. Therefore wildtype E.coli (MC4100) and its isogenic crl::cat mutant were grown at 30oC. Total RNA was extracted at on OD (578nm) of 4 (during entry into stationary phase) and then the analysis proceeded as described in detail in the protocoles. The experiment was repeated three times and the results from those experiments are presented here.

INSTRUMENT(S): GenePix Personal 4100A [Axon Instruments]

ORGANISM(S): Escherichia coli

SUBMITTER: Athanasios Typas 

PROVIDER: E-MEXP-720 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Stationary phase reorganisation of the Escherichia coli transcription machinery by Crl protein, a fine-tuner of sigmas activity and levels.

Typas Athanasios A   Barembruch Claudia C   Possling Alexandra A   Hengge Regine R  

The EMBO journal 20070301 6

Upon environmental changes, bacteria reschedule gene expression by directing alternative sigma factors to core RNA polymerase (RNAP). This sigma factor switch is achieved by regulating relative amounts of alternative sigmas and by decreasing the competitiveness of the dominant housekeeping sigma(70). Here we report that during stationary phase, the unorthodox Crl regulator supports a specific sigma factor, sigma(S) (RpoS), in its competition with sigma(70) for core RNAP by increasing the formati  ...[more]

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