Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

25 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of potent induction of flowering in A. thaliana by elevated temperature

ABSTRACT: Plants were grown on soil for 35 days under short day conditions (8hrs light/16hrs dark) at 16¡ÄC and 65% relative humidity. Flowering was induced by shifting either to long day conditions (16/8) at 16¡ÄC and 65% relative humidity or to 25¨¬C in short days (8/16). Samples were taken at day 0, 2, 5 and 9 after shifting.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Sridevi Sureshkumar

PROVIDER: E-MEXP-728 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Potent induction of Arabidopsis thaliana flowering by elevated growth temperature.

Balasubramanian Sureshkumar S Sureshkumar Sridevi S Lempe Janne J Weigel Detlef D

PLoS genetics 20060526 7

The transition to flowering is an important event in the plant life cycle and is modulated by several environmental factors including photoperiod, light quality, vernalization, and growth temperature, as well as biotic and abiotic stresses. In contrast to light and vernalization, little is known about the pathways that mediate the responses to other environmental variables. A mild increase in growth temperature, from 23 degrees C to 27 degrees C, is equally efficient in inducing flowering of Ara ...[more]

PMID: 16839183

Similar Datasets

H2A.Z - Containing Nucleosomes Mediate the Thermosensory Response in Arabidopsis

Project description:We isolated mutants in Arabidopsis with enhanced ambient temperature response. Microarray analysis was performed to understand the extent to which ambient temperature transcriptome is perturbed in the mutants in comparison with the WT at non inductive 12 ºC and after shift to inductive 27 ºC for 2 h and 24 h. We performed global transcript profiling to understand the regulation of of gene expression underlying ambient temperature response. Sterilized wild type Arabidopsis Col-0 as well as the arp6-10 mutant seeds were plated on half-strength MS plates without sugar. Plates were incubated at 4 ºC in dark for 48 hours before transfering to 20 ºC with light. 5 day old seedlings were transfered to 12 ºC incubaor with continuous light for 3 days befroe shifting to 27 ºC. Samples (Whole seedlings) were collected before shifting (0 h) and 2 h and 24 h after shifting to 27 ºC by immediately freezing in liquid nitrogen.

2010-01-08 | E-GEOD-18624 | biostudies-arrayexpress

Transcription profiling of Arabidopsis plants of three different genotypes (FRI FLC, FRI flc and fri flc) induced to flowering by shifting from short day conditions to long day conditions

Project description:Plants of three different genotypes (FRI FLC, FRI flc and fri flc) were induced to flowering by shifting from short day conditions to long day conditions. FRI=FRIGIDA, FLC=FLOWERING LOCUS C.

2005-09-08 | E-TABM-19 | biostudies-arrayexpress

Transcription profiling of Arabidopsis penetration resistance, wildtype and ataf1-1 mutant response to Bgh 12 h after inoculation, 2x2 factorial design

Project description:Arabidopsis is a non-host to the biotrophic fungus Blumeria graminis f.sp. hordei (Bgh), effectively hindering Bgh ingress to the epidermal cells by deposition of callosic papillae formations, i.e. penetration resistance. To better understand the transcriptional changes in Arabidopsis towards Bgh, we compared un-inoculated and inoculated wild-type Arabidopsis samples, with those an ATAF1 mutant allele, compromised in penetration resistance. The experiment included sampling of 8 rosettes for each replicate sample from 6-weeks old plants, 12 hrs after inoculation. A total of 12 biolocigal replicates were sampled with three replicates from each of the four blocks (Wild-type, ataf1-1, Bgh, control). ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Michael Krogh Jensen. The equivalent experiment is AT31 at PLEXdb.] Experiment Overall Design: genotype: Wild-type Arabidopsis, ecotype Col-0 - treated or untreated: Un-inoculated(3-replications); genotype: Wild-type Arabidopsis, ecotype Col-0 - treated or untreated: Bgh inoculated(3-replications); genotype: ataf1-1 mutant, ecotype Col-0 - treated or untreated: Un-inoculated(3-replications); genotype: ataf1-1 mutant, ecotype Col-0 - treated or untreated: Bgh inoculated(3-replications)

2008-10-25 | E-GEOD-12856 | biostudies-arrayexpress

Schmid_flowering_mutants_2013

Project description:Synchronized induction of flowering in Arabidopsis thaliana wild type (Col-0) and flowering time mutants (soc1, agl24, fd) by shifting from short day (8 hr light, 16 hr dark; 23C; 65% rel humidity) to long day (16 hr light, 8 hr dark; 23C; 65% rel humidity) for 0, 3, 5, and 7 days. Biotinylated probes were synthesized from RNA isolated from manually disseted shoot meristems and hybridized to Affymetrix ATH1 arrays.

2013-11-01 | E-MEXP-4001 | biostudies-arrayexpress

Expression profiling of Fusarium oxysporum infected wild-type Arabidopsis and a 35S Fusarium SIX4 overexpression line.

Project description:Fungal effectors play important roles in inciting disease development on host plants. We identified an effector (Secreted in Xylem4, SIX4) in an Arabidopsis infecting isolate (Fo5176) of the root-infecting fungal pathogen Fusarium oxysporum and demonstrated this effector is required for full virulence. To explore the role of Fo5176_SIX4 we use whole transcriptome profiling of root tissues from plants overexpressing this effector (35sSIX4) versus wild-type (Col-0) plants after F. oxysporum infection. We grew both WT and 35sSIX4 plants for four weeks in soil. After four weeks the plants were infected with Fusarium oxyporum isolate Fo5176, trays covered with a plastic dome and incubated at 28C. There were four independent replicates of each treatment and each replicate contained root tissue from 20 plants. Each replicate (8 in total) was harvested 4 days post inoculation and the resulting RNA was used for hybridization to an Affymetrix ATH1 chip.

2016-05-31 | E-GEOD-75928 | biostudies-arrayexpress

short-term effect of N and S supply shifts on gene expression in wheat grain

Project description:Nitrogen and Sulfur supply play an important role in modulating wheat grain development. We studied the short-term effect of shifting N and S supply levels at mid-way through grain filling on gene expression by microarray analysis. Please note: Factor Value[sampling time]: indicates when the samples were sampled, e.g. 3 hours after we shift the treatment as explained in the protocol TREATMENT and indicated in the protocol SAMPLING. Factor Value[growth condition] (a, b, c, d, e, f) corresponds to the treatments explained in the protocol TREATMENT: a) 3mM nitrogen and 0.02mM sulfur; b) 15mM nitrogen and 0.02mM sulfur; c) 3mM nitrogen and 2mM sulfur. At mid-way through grain filling, three additional N and S supply levels were introduced as follows: d) shifting treatment a) to 15mM nitrogen and 0.02mM sulfur; e) shifting treatment b)to 15mM nitrogen and 2mM sulfur; f) shifting treatment c) to 15mM nitrogen and 2mM sulphur.

2015-06-01 | E-MTAB-1920 | biostudies-arrayexpress

Effect of auxin signalling on flg22 response

Project description:Expression data after flg22 treatment on leaf discs in Col-0, 35S:AFB1 and 35S:miR393 We used microarrays to investigated the effect of auxin signalling on flg22 response pathway. We also compared if addition of auxin could simulated the 35S:AFB1 phenotype. Keywords: stress response, disease state analysis Leaf discs were taken and let overnight in water to avoid the wound response. We then performed the treatment (flg22, flg22 from Agrobacterium tumefaciens, flg22 +NAA, NAA) to the media. The samples where harvested and freeze in liquid nitrogen at 0, 1 and 2h after treatment.

2009-10-31 | E-GEOD-17479 | biostudies-arrayexpress

Transcription profiling of Arabidopsis thaliana LFY mutant plants transformed with either Arabidopsis LFY or Leanworthia crassa LFY to investigate evolutionary divergence of LFY function

Project description:The aim of this experiment is to test the ability of the ortholog of Arabidopsis LFY gene from Leanworthia crassa (Lcr) to complement an Arabidopsis LFY mutant. Plants used are homozygous lfy6 mutants (EMS alleles) in Ler background which are transformed or not (for the lfy6 mutant) by genomic clones for Arabidopsis LFY (AthLFY) or Leanworthia crassa LFY (LcrLFY). Flowering was synchronized by growing plants in SD then shifting them to LD. 2 time points samples (wild type Ler) were taken at the end of the SD period as a reference for genes induced by shifting to LD, irrespective of the status at the LFY locus.

2006-08-17 | E-MEXP-682 | biostudies-arrayexpress

Cold acclimation in the sfr3-1 mutant

Project description:The sfr3-1 mutation causes freezing-sensitivity in Arabidopsis thaliana. The mutated gene has been identified by positional cloning and is currently being characterised. The mutant appears normal when grown in the warm (no phenotype has been identified associated with such growth). However, following cold acclimation and subsequent freezing mutant plants are severely damaged whilst wild type plants are not. This suggests that sfr3 is deficient in the cold acclimation process. Micro-array analysis will enable the identification of any transcriptional changes during the cold acclimation process. This information will then be used, together with information obtained by gene characterisation, in order to more fully understand the nature of the sfr3 mutation. 8 samples were used in this experiment.

2009-12-03 | E-GEOD-19254 | biostudies-arrayexpress

Small RNAs from the lycopod Selaginella moellendorffii

Project description:The small RNAs of Selaginella moellendorffii were sampled by direct pyrosequencing (454 Life Sciences). These data represent microRNAs, siRNAs, and other expressed small RNAs of ~18-~28 nts. in length. Keywords: Small RNA sequencing - high throughput One sample of above-ground tissues of greenhouse-grown S. moellendorffii was used to extract and sequence small RNAs.

2007-06-29 | E-GEOD-7320 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data