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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human CD4 T cell subsets isolated from peripheral blood and palatine tonsils

ABSTRACT: Comparatative gene expression analysis for CD4 T cell subsets isolated from peripheral blood and palatine tonsils

ORGANISM(S): Homo sapiens

SUBMITTER: Gerd Müller

PROVIDER: E-MEXP-750 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A methodology for global validation of microarray experiments.

Miron Mathieu M Woody Owen Z OZ Marcil Alexandre A Murie Carl C Sladek Robert R Nadon Robert R

BMC bioinformatics 20060705

<h4>Background</h4>DNA microarrays are popular tools for measuring gene expression of biological samples. This ever increasing popularity is ensuring that a large number of microarray studies are conducted, many of which with data publicly available for mining by other investigators. Under most circumstances, validation of differential expression of genes is performed on a gene to gene basis. Thus, it is not possible to generalize validation results to the remaining majority of non-validated gen ...[more]

PMID: 16822306

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Long non-coding RNA HOTTIP promotes CCL3 expression and induces cartilage degradation by sponging miR-455-3p

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2019-08-12 | PXD014988 | Pride

Single cell RNAseq of immune and stromal cells from human palatine tonsils

Project description:Palatine tonsils are secondary lymphoid organs that are strategically positioned in the oropharynx to secure a first line of defense against oral pathogens. Specialized immune-interacting fibroblasts, generally termed fibroblastic reticular cells (FRC), underpin distinct microenvironments within lymphoid organs to compartmentalize and direct the efficient interaction and activation of immune cells. As a particular anatomical property, palatine tonsils harbor a reticular-shaped lymphoepithelium that generates an antigen sampling zone in the crypts. While the histological ultrastructure and the immune cell composition of human palatine tonsils has been elaborated in detail, the molecular identity of the diverse stromal cell compartments including FRC and the specialized lymphoepithelium remains largely unknown. Here, we have employed single cell transcriptomics and extensive flow cytometric analyses to unveil the molecular identity of tonsillar cells and to disentangle the heterogeneity of fibroblast and epithelial cell subsets in palatine tonsils. Our results reveal a remarkable conservation of stromal cell organization and molecularly-defined subsets in infant and adult human palatine tonsils.

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lnc-EAPV binding proteins in C57BL/6J BMDMs

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