Transcription profiling of human BJ fibroblasts after LIN-9 depletion by a shRNA

ABSTRACT: Aim: identification of differentially expressed genes after LIN-9 depletion.

hTERT immortalized human BJ fibroblasts were infected with pMSCV-Blasticidin based retroviruses encoding a shRNA which targets human LIN-9 and the empty vector respectively.
After 4 days of selection total RNA was isolated. Cy3 and Cy5 labelled cDNA probes were generated using the CyScribe Post-labelling Kit (Amersham Biosiences).

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens

SUBMITTER: Stefanie Hauser 

PROVIDER: E-MEXP-752 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress