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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse embryonic stem cell differentiation following treatment with DKK1

ABSTRACT: Assessed effect of DKK1 treatment on gene expression during the first four days of ES cell differentiation in serum containing medium.

ORGANISM(S): Mus musculus

SUBMITTER: R. Coleman Lindsley

PROVIDER: E-MEXP-759 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcription factors T-bet and GATA-3 control alternative pathways of T-cell differentiation through a shared set of target genes.

Jenner Richard G RG Townsend Michael J MJ Jackson Ian I Sun Kaiming K Bouwman Russell D RD Young Richard A RA Glimcher Laurie H LH Lord Graham M GM

Proceedings of the National Academy of Sciences of the United States of America 20091005 42

Upon detection of antigen, CD4(+) T helper (Th) cells can differentiate into a number of effector types that tailor the immune response to different pathogens. Alternative Th1 and Th2 cell fates are specified by the transcription factors T-bet and GATA-3, respectively. Only a handful of target genes are known for these two factors and because of this, the mechanism through which T-bet and GATA-3 induce differentiation toward alternative cell fates is not fully understood. Here, we provide a geno ...[more]

PMID: 19805038

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Transcription profiling of mouse ES cells bearing a targeted, doxycycline inducible Mesp1 transgene were differentiated in the absence or presence of DKK1

Project description:During gastrulation, cells of the prospective mesoderm ingress through the primitive streak and acquire fates based on complex spatial and temporal cues. Progenitors of the cardiogenic mesoderm are first found at E6.5 in the posterior lateral epiblast and subsequently migrate laterally and anteriorly to form the cardiac crescent at E7.5, when regionalized cell fates are first delineated . Lineage tracing and heterotopic transplantation studies suggest that precursors in the earliest heart field possess potential to generate myocardium, endocardium, and pericardium. The mechanisms by which inductive signals in the primitive streak effect the development of this pancardiac progenitor field, however, remain poorly understood; In mice, the earliest restricted progenitors of the cardiovascular system are marked by expression of the basic helix-loop-helix transcription factor, Mesp1. We therefore use microarray analysis to determine the early and intermediate effects of transiently forced Mesp1 expression during ES cell differentiation. Experiment Overall Design: ES cells bearing a targeted, doxycycline inducible Mesp1 transgene were differentiated in the absence or presence of DKK1 from day 0-4, either with or without transient doxycycline treatment from day 2-4.

2008-10-18 | E-GEOD-5976 | biostudies-arrayexpress

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