Project description:Response of C. elegans to Serratia marcesens infection at 24 hours. This submission is not MIAME compliant as only a subset of each raw data file has been supplied by the submitter
Project description:Response of C. elegans to Serratia marcesens infection at 48 hours.This submission is not MIAME compliant as only a subset of each raw data file has been supplied by the submitter.
Project description:Response of C. elegans to Drechmeria coniospora infection at 24 hours. This submission is not MIAME compliant as only a subset of each raw data file has been supplied by the submitter.
Project description:Response of C. elegans to infection by Drechmeria coniospora at 12 hours. This submission is not MIAME compliant as only a subset of each raw data file has been supplied by the submitter.
Project description:Transcritome study of C.elegans exposed to multiple, different bacterial pathogens. Experiments were performed in set-replicates of either 3 or 5.<br> There are 3 for samples: Aeromonas hydrophila, Enterococcus faecalis, Erwinia carotovora and Photorhabdus luminescens. <br> There are 5 for samples: Serratia marcesens and Escherichia coli (control).<br>
Project description:1.Sample Growth Conditions protocols<br> Eggs from IG274 wt; frIs7 and IG339 nipi-1(fr1); frIs7 worms cultivated at 25ðC were allowed to hatch in the absence of food at 25ðC for 16 hours. Synchronised larvae were transferred to NGM agar plates lawned with OP50 and cultivated at 25°C for 48 hours.<br> 2.Sample Treatment protocols <br> Synchronised IG274 wt; frIs7 and IG339 nipi-1(fr1); frIs7 worms at the young L4 stage were infected with freshly harvested spores of Dreshmeria coniosporia. After 24 hours at 25°C, the worms were collected into trizol for further RNA extraction. Controls worms were non infected.