Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

14 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of QMP pheromone-mediated gene expression in the honey bee brain

ABSTRACT: This experiment was designed to monitor the gene expression changes of young bees raised with and without queen mandibular pheromone (QMP). This experiment was a timecourse, comparing QMP- to QMP+ bees over 4 days of exposure. Bees that had eclosed over 16 hours were collected from a brood frame and placed in cages, 35 bees/cage. 8 hours later, queen mandibular pheromone (QMP, 0.1 queen equivalents) treatment was initiated, and fresh QMP was placed in the cage every day. One hour after QMP treatment had been refreshed, QMP- and QMP+ cages were collected into liquid nitrogen. 8 cages of each were collected on each day of the timecourse (1 day of treatment = Day 1, as well as Day 2, Day 3, and Day4).

ORGANISM(S): Apis mellifera

SUBMITTER: Noura Sharabash

PROVIDER: E-MEXP-79 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Pheromone-mediated gene expression in the honey bee brain.

Grozinger Christina M CM Sharabash Noura M NM Whitfield Charles W CW Robinson Gene E GE

Proceedings of the National Academy of Sciences of the United States of America 20031022

We tested the hypothesis that queen mandibular pheromone (QMP) causes changes in gene expression in the brain of the adult worker honey bee, and that these changes can be correlated to the downstream behavioral responses induced by QMP. In support of the first hypothesis, cage experiments revealed that QMP transiently regulated expression of several hundred genes and chronically regulated the expression of 19 genes. Several of these genes were also affected by QMP in experiments with bee colonie ...[more]

PMID: 14573707

Similar Datasets

Transcription profiling of young bees raised in colonies with a queen, with queen mandibular pheromone (QMP), or without a queen.

Project description:This experiment was designed to identify gene expression differences in young bees raised in colonies with a queen, with queen mandibular pheromone (QMP), or without a queen. The source colony was split into three colonies, allocating roughly equal quantities of adult bees, brood, and pollen and nectar stores. One colony retained the original queen (QR), one was left queenless (QL), and the third was given a strip that contained 10 Qeq (QMP+), a dose shown to mimic a live queen (20). The three colonies were transferred to a different apiary > 2 mi away so they would not return to the site of their natal hive. Prior to the colony split, bees (N ~1500) were collected 0-36 h after eclosion, marked on the dorsal thorax with a paint dot (Testors Paint), and ~500 were placed in each of the three colonies. Two days later, the marked bees were collected (N = 100). Bees were collected into liquid nitrogen, and heads were stored as above.

2004-10-15 | E-MEXP-80 | biostudies-arrayexpress

Transcription profiling of brains of free-flying forager bees and hive-restricted bees apparently attempting to forage

Project description:Expression profiling of brains of free-flying forager bees and hive-restricted bees that rushed toward the hive entrance when the screen was removed, apparently to attempt to forage.

2007-04-21 | E-TABM-151 | biostudies-arrayexpress

Transcription profiling of brains from honey bees of different ages, subspecies and host colony environment

Project description:Expression profiling of honey bee brains as a function of age, genotype (subspecies differences) and environment (host colony)

2007-04-25 | E-TABM-149 | biostudies-arrayexpress

Transcription profiling of honey bee CNS regions in 3 species during dance communication behavior

Project description: Not available

2009-07-29 | E-TABM-700 | biostudies-arrayexpress

Transcription profiling of brains of adult honey bee workers (Apis mellifera) performing different behavioral tasks in the hive

Project description:This experiment examines gene expression profiles in the brains of adult honey bee workers (Apis mellifera) performing different behavioral tasks in the hive. The different behavioral groups examined were nurse, comb builder, guard, undertaker, and forager. The comb builder, guard, and undertaker behavioral groups perform their respective tasks over a relatively short time scale (typically 1 day), while nursing and foraging are longer duration (lasting > 1 week). The purpose of this study was to examine whether behaviors that persist over different time scales are associated with differences in the extent of gene expression changes in the brain.

2005-01-21 | E-MEXP-252 | biostudies-arrayexpress

Regulation of Brain Gene Expression in Honey Bees by Queen Mandibular Pheromone

Project description:Expression profiling of honey bee brains exposed to queen mandibular pheromone. Exposure was performed in cages for 3 and 4 days; 3 and 4 days-old bees were analyzed

2011-09-01 | E-TABM-1102 | biostudies-arrayexpress

Mechanisms of Stable Lipid Loss in a Social Insect

Project description:Effects of behavioral maturation, diet quality and Queen Mandibular Pheromone on gene expression in the abdominal fat bodies of worker honey bees.

2011-07-01 | E-MTAB-495 | biostudies-arrayexpress

Transcription profiling of honey bee brain and ovary reveals pheromone response is correlated with reproductive traits and brain gene expression in individual honey bees

Project description:In honey bees (Apis mellifera), the reproductive queen produces a pheromonal signal that regulates many aspects of worker behavior and physiology and is critical for maintaining colony organization. Queen mandibular pheromone (QMP) inhibits worker reproduction, attracts workers from a short distance (retinue response), inhibits the rearing of new queens, modulates age-related division of labor and globally alters brain gene expression in worker bees. Interestingly, substantial variation in worker retinue responses to QMP has been found between colonies, but the molecular and physiological bases for variation in individual responses to the queen have not been characterized. Here, we demonstrate that individual retinue response is negatively correlated with traits associated with reproductive potential. Workers with low response to QMP have more ovarioles and higher levels of vitellogenin transcripts than workers with a high response to QMP, suggesting that workers with greater reproductive potential may be attempting to escape queen control. Retinue response appears to be associated with a suite of behavioral and physiological traits that may be pleiotropically linked. However, while these phenotypes are all correlated at the organismal level, the underlying brain expression patterns and gene networks associated with each trait are independent, suggesting that these phenotypes are uncoupled at the molecular level in adult bees. These studies provide insights into the ultimate and proximate causes of natural variation in pheromone response in honey bees.

2008-12-31 | E-MEXP-1724 | biostudies-arrayexpress

Transcription profiling of brains of 10 day old virgin queen, sterile worker, and reproductive worker honey bees

Project description:We used microarrays to monitor expression patterns of several thousand genes in the brains of same-aged (10 day old) virgin queens, sterile workers, and reproductive workers in honey bees (Apis mellifera).

2008-07-29 | E-MEXP-1044 | biostudies-arrayexpress

Parallel Epigenomic and Transcriptomic Responses to Viral Infection in Honey Bees (Apis mellifera)

Project description:Here, we examined the transcriptional and epigenetic (DNA methylation) responses to viral infection in honey bee workers. One-day old worker honey bees were fed solutions containing Israeli Acute Paralysis Virus (IAPV), a virus which causes muscle paralysis and death and has previously been associated with colony loss. Uninfected control and infected, symptomatic bees were collected within 20-24 hours after infection. Worker fat bodies, the primary tissue involved in metabolism, detoxification and immune responses, were collected for analysis. We performed transcriptome- and bisulfite-sequencing of the worker fat bodies to identify genome-wide gene expression and DNA methylation patterns associated with viral infection. There were 753 differentially expressed genes (FDR<0.05) in infected versus control bees, including several genes involved in epigenetic and antiviral pathways. DNA methylation status of 156 genes (FDR<0.1) changed significantly as a result of the infection, including those involved in antiviral responses in humans. There was no significant overlap between the significantly differentially expressed and significantly differentially methylated genes, and indeed, the genomic characteristics of these sets of genes were quite distinct. Our results indicate that honey bees have two distinct molecular pathways, mediated by transcription and methylation, that modulate protein levels and/or function in response to viral infections. Examination of epigenomic and transcriptomic antiviral responses to Israeli Acute Paralysis Virus in honey bees

2015-02-06 | E-GEOD-65659 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data