Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of wheat roots from drought-tolerant wheat after drought and normal watering treatments

ABSTRACT: Plant material consisted of synthetic hexaploid wheat germplasm into the Opata background (Altar 84/ Aegilops squarrosa (TAUS)//Opata) . Plants were grown at a density of 9-11 individuals per 20cm x 10cm (diameter x height) plastic pot containing 1500g well-rinsed Turface MVP® medium (Profile Products LLC, Buffalo, IL), in controlled environment chambers at 23°C, 70% relative humidity, and 16h photoperiod with a photosynthetic photon flux (PPF) of 330±10 µmolem-2s-1 when measured at the top of the canopy at growth stage 22 to 24 in the Zadoks scale (Zadoks et al., 1974). Plants were watered daily until 21 days after seeding (DAS) by flooding trays with water for 5 minutes, then draining the trays. Drought stress was applied by water withholding, beginning on 21 DAS. Watering was withheld from plants belonging to drought treatment, while the control group received water above field capacity. Water content of the growth medium was gravimetrically monitored. Root tissues were collected from control and treated plants when the water content of the treatment group growth medium fell below the permanent wilting point. Three biological replicates were conducted in three separate time-span courses. Total RNA was isolated using a LiCl3 precipitation method following Moore et al., (2005). Dye swap desin microarray analyses of well-watered and water-stressed root tissues were conducted across three biological replicates totaling six hybridizations.

INSTRUMENT(S): arrayWoRx Biochip Reader [Applied Precision]

ORGANISM(S): Triticum aestivum

SUBMITTER: Mohsen Mohammadi

PROVIDER: E-MEXP-831 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptional profiling of hexaploid wheat (Triticum aestivum L.) roots identifies novel, dehydration-responsive genes.

Mohammadi Mohsen M Kav Nat N V NN Deyholos Michael K MK

Plant, cell & environment 20070501 5

We used a long-oligonucleotide microarray to identify transcripts that increased or decreased in abundance in roots of dehydration-tolerant hexaploid bread wheat, in response to withholding of water. We observed that the major classes of dehydration-responsive genes (e.g. osmoprotectants, compatible solutes, proteases, glycosyltransferases/hydrolases, signal transducers components, ion transporters) were generally similar to those observed previously in other species and osmotic stresses. More s ...[more]

PMID: 17407540

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Dataset Information

Transcription profiling of wheat roots from drought-tolerant wheat after drought and normal watering treatments

Publications

Transcriptional profiling of hexaploid wheat (Triticum aestivum L.) roots identifies novel, dehydration-responsive genes.

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