Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis cell suspension cultures treated with salicylic acid or wortmannin

ABSTRACT: Cell suspension culture of Arabidopsis thaliana (ecotype Columbia) was treated by 250 mM salicylic acid and by two different concentrations of wortmannin (1 mM and 30 mM) for 4 hours.

INSTRUMENT(S): Axon GenePix 4000A scanning hardware

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: ludivine taconnat

PROVIDER: E-MEXP-837 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Experiment 1 Gene profiling upon biotic stress treatment Biological question : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? Experiment description : 14-day old seedlings (Col-0 and fls2 KO) treated with 10uM of flg22 (flagellin- derived peptide) over a one hour timecourse. Experiment 2 Gene profiling in microRNA mutants Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? Experiment description : Ler, dcl1-9, hen1-1 and hasty were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after. Experiment 3 Gene profiling in siRNA mutants Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? Experiment description : Col-0, dcl2-1, dcl3-1 and rdr2-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after. Experiment 4 Gene profiling in Agrobacterium-induced tumors and auxin-induced calli. Biological question : What are the genes (including miRNA precursors) that are differentially regulated in an Agrobacterium-induced tumors compared to auxin-induced calli? Experiment description : Col-0 plants were infected with wt Agrobacterium tumefaciens (strain A208) by stabbing the emerging stems. 18 days later, growing tumors were harvested as were the non-infected part of the stabbed-stem.In parallel, Col-0 seeds were germinated on Callus Induction Media (CIM) and 18 days later, calli were harvested. Experiment 5 Gene profiling in tasiRNA mutants Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of tasiRNA mutants? Experiment description : Col-0, dcl4-1, rdr6-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after. Experiment 6 Gene profiling upon biotic stress treatment Biological question : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? Experiment description : 14-day old seedlings (Col-0 and fls2 KO) treated with 10uM of flg22 (flagellin- derived peptide) over a one hour timecourse.

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Transcription profiling of Arabidopsis cell suspension cultures treated with salicylic acid or wortmannin

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