Transcription profiling of Ectocarpus strain Esil32 porophyte and gametophyte stages in normal and mutant strains to analyse expression of two sets of genes previously identified as being putatively sporophyte-specific or gametophyte-specific

ABSTRACT: The aim of this experiment was to analyse the expression of two sets of genes identified as being putatively sporophyte-specific or gametophyte-specific by a suppressive subtraction hybridisation using cDNA from immature sporophytes and immature gametophytes of the Ectocarpus strain Esil32. The expression of these genes was analysed in the sporophyte and gametophyte generations of the life cycle (again using immature algae that had not yet produces zoidangia) and in the sporophyte generation of a mutant strain, immediate upright, that exhibits gametophyte-like characteristics during the sporophyte generation.

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Ectocarpus siliculosus

SUBMITTER: J. Mark Cock 

PROVIDER: E-MEXP-848 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress