Chromosome interaction copy number analysis of mouse neonatal liver cells, and undifferentiated and differentiated embryonic stem cells using circular chromosome conformation capture (4C) assays

ABSTRACT: Experimental design
• Using Circular chromosome conformation capture (4C) assay to uncovers epigenetically regulated intra- and inter-chromosomal interaction network
• To examine restrictions imposed by the nuclear structure on such cis/trans-chromosomal networks, we developed a high-throughput screening assay 4C and identified 114 different sequences from all autosomes in mouse liver cells. To check the relative frequencies of interactions, the 114 sequences were PCR amplified and spotted on glass to make dedicated microarray. The microarray and 3C validation showed that most of the sequences interact with primarily the maternally inherited H19 imprinting control region (ICR). The epigenetic feature of these interactions was highlighted by the observation that 19% of 4C library se

INSTRUMENT(S): ScanArray 4000 [PerkinElmer]

ORGANISM(S): Mus musculus

SUBMITTER: gholamreza tavooisdana 

PROVIDER: E-MEXP-870 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress