Transcription profiling of fibroblast-like synovial cells from normal adult horse following treatment with hyaluronan and challenged with lipopolysaccharide

ABSTRACT: Greater than 95% confluent (Day 0) fibroblast-like synovial cells from the hind limbs (pelvic limb) of normal adult horses were allocated in triplicate to one of 4 groups:

(group 1) unchallenged;
(group 2) LPS-challenged;
(group 3) LPS-challenged following pre-treatment and sustained treatment with lower (5 - 7.5 x 105 Da) molecular weight HA (Bioniche Animal Health, Pullman, WA) at 10 mg/ml;
(group 4) LPS-challenged following pre-treatment and sustained treatment with higher (3 x 106 Da) molecular weight HA (Pfizer Animal Health, New York, NY) at 5 mg/ml.

Microarray data were analyzed using dChip version 1.3 (Harvard University, Cambridge, MA). Array normalization was performed using the invariant set procedure; model-based expression indices (MBEI) wer

ORGANISM(S): Equus caballus

DISEASE(S): normal

SUBMITTER: Kelly Santangelo 

PROVIDER: E-MEXP-940 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress