Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

10 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of callus, petals and stamens from wild type and gmyb10 over-expressing gerbera plants in order to identify target genes for gmyb10 in the anthocyanin pathway

ABSTRACT: In the microarray experiment a comparison of different flower organs of transformed transgenic gerbera line to wild type gerbera was done. In the transgenic gerbera line the gene gmyb10 that regulates anthocyanin pathway is over-expressed. In this line anthocyanin pigmentation is altered compared to the wild type. In this experiment RNA from dark red callus, two stage of petal development and stamens of the transgenic lines were compared to RNA of wild type in order to identify target genes for gmyb10.

INSTRUMENT(S): ScanArray scanning hardware

ORGANISM(S): Gerbera hybrid cultivar

SUBMITTER: Roosa Laitinen

PROVIDER: E-MEXP-959 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida.

Laitinen Roosa A E RA Ainasoja Miia M Broholm Suvi K SK Teeri Teemu H TH Elomaa Paula P

Journal of experimental botany 20080825 13

Genetic modification of the flavonoid pathway has been used to produce novel colours and colour patterns in ornamental plants as well as to modify the nutritional and pharmaceutical properties of food crops. It has been suggested that co-ordinate control of multiple steps of the pathway with the help of regulatory genes would lead to a more predictable control of metabolic flux. Regulation of anthocyanin biosynthesis has been studied in a common ornamental plant, Gerbera hybrida (Asteraceae). An ...[more]

PMID: 18725377

Similar Datasets

Transcription profiling of Gerbera-Ray vs Disc flowers

Project description:Three different developmental stages of ray and disc flowers of gerbera hybrida were compared to each other using gerbera 9K cDNA microarray.

2005-12-31 | E-MEXP-418 | biostudies-arrayexpress

Transcription profiling of Gerbera hybrida flower organs vs a reference pool

Project description:Different gerbera hybrida flower organs including pappus bristles, stamen, early and late petal development and flower scape, were compared to a pooled reference sample to determine which genes are abundant for analyzed flower organs. Samples were pooled and three replicants that were independently labelled were included. All labellings/hybridizations included one dye swap technical repat. Gerbera 9K cDNA microarrays were used for hybridizations.

2004-12-31 | E-MEXP-134 | biostudies-arrayexpress

Transcription profiling of Gerbera hybrida flower organs vs a reference pool

Project description:Different gerbera hybrida flower organs including pappus bristles, stamen, early and late petal development and flower scape, were compared to a pooled reference sample to determine which genes are abundant for analyzed flower organs. Samples were pooled and included two biological replicants with three technical replicants that were independently labelled were included. Each biological replicant included one dye swap technical repeat. Gerbera 9K cDNA microarrays were used for hybridizations.

2004-12-31 | E-MEXP-207 | biostudies-arrayexpress

Transcription profiling of Gerbera hybrida flower organs vs leaf

Project description:Gerbera inflorescence sample was compared with a gerbera leaf sample in order to identify flower specific genes. Samples were pooled from different developmental stages and 4 replicants were independently labelled. All labellings/hybridizations included two dye swap replicants. Gerbera 9K cDNA microarrays were used for the hybridizations.

2004-12-31 | E-MEXP-133 | biostudies-arrayexpress

Transcription profiling of Gerbera inflorescence sample was compared with a gerbera leaf sample

Project description:Gerbera inflorescence sample was compared with a gerbera leaf sample in order to identify flower specific genes. Samples were pooled from different developmental stages and alltogether 8 replicates (including 2 biological replicates with 4 technical replicates each) were independently labelled. Both biological replicates included two dye swap replicates. Gerbera 9K cDNA microarrays were used for the hybridizations.

2004-12-31 | E-MEXP-206 | biostudies-arrayexpress

Transcription profiling of Gerbera petals of developmental stages 2, 4, 6, 7, 8, and 9 vs a reference sample to identify transcriptional changes in petal development

Project description:Gerbera petals of inflorescence developmental stages 2, 4, 6, 7, 8, and 9 were compared against a reference sample that included same amount of mRNA from stages 1-11. Alltogether six comparisons were done each including two biological replicates with 3 technical replicates resulting alltogether 36 hybridizations. At least one of each biological replicate was dyeswap hybridization. This experiment was done in order to profile transcriptional changes that occur in petals from stage where they still divide until senescence of the cells. Developmental stages have been characterized by Helariutta et al. (1995).

2006-09-01 | E-MEXP-578 | biostudies-arrayexpress

Transcription profiling of tomato strains during fruit set

Project description:Transcriptome profiling of tomato gene expression during fruit set

2009-05-06 | E-MEXP-1617 | biostudies-arrayexpress

EST sequencing and microarray analysis of the floral transcriptome of Eustoma grandiflorum

Project description:Genome-wide DNA sequence resources are expected to enhance our understanding of the molecular basis of plant development and lead to improvements in desirable traits in horticultural crops. In this study, we sequenced 643,366 ESTs from Eustoma grandiflorum flowers using a normalised cDNA library constructed for several different flower development stages, times of day, and pollinated stamens. The sequences were assembled into 63,401 contigs and 242,212 singletons. BlastX searches for all of the contigs in the GenBank database matched 65% of the contigs to registered sequences, while 35% presented no hits. GO mapping assigned 48% of the 63,401 contigs to GO terms. Microarray analysis showed that subsets of genes were up- or downregulated as the flower developed. The downregulated genes were enriched for GO terms related to 1) oligopeptide transport, 2) response to jasmonic acid stimulus, and 3) cell wall modification, whereas the upregulated genes were enriched for GO terms involved in 1) secondary metabolism, such as the flavonoid biosynthetic process and the terpenoid biosynthetic process, and 2) epidermal cell modification. Two-condition experiment, loop-design among 4 developmental stages. Biological replicates: 4.

2012-09-01 | E-GEOD-34332 | biostudies-arrayexpress

Taura Syndrome Virus or Yellow Head Virus infection in two strains of L. vannamei different in their resistance to TSV

Project description:The transcriptomic response of two strains of the Pacific whiteleg shrimp, different in their resistance to Taura Syndrome Virus (TSV), in response to infection with TSV and Yellow Head Virus (YHV). Changes in gene expression in the shrimp’s hepatopancreas were assessed using a cDNA microarray containing 2,469 putative unigenes. The patterns of gene expression between the shrimp strains were considerably similar, except for the more advanced stages of Taura Syndrome. Between the different treatments approximately 250 genes were differently expressed. The most advanced stages of YHV infection showed the highest number of differently expressed genes. During infection there were profound changes in the expression of genes related to lipid and protein metabolism, cellular trafficking, immune defense and stress response. Keywords: Disease state analysis, disease resistance There were 5 biological replicates for each of the groups in this experiment. Also, two strains of Litopenaeus vannamei were used: a strain resistant to TSV and a strain susceptible to TSV (Kona line). The treatments consisted of injecting both strains with 60mL of a shrimp extract made from shrimp previously injected with either a SPF shrimp extract (1x10-4), Taura Syndrome Virus (1x10-5) or Yellow Head Virus (1x10-4). The 2 initial control groups were composed of hepatopancreas samples from both strains prior the injections. Samples were also collected from at days 1 and 2 from both strains from the 3 different treatments (control, TSV and YHV).

2009-10-23 | E-GEOD-13354 | biostudies-arrayexpress

Transcription profiling of brain from homozygous narcoleptic dogs and their heterozygous unaffected siblings

Project description:Brain gene expression profiles of homozygous narcoleptic dogs and their heterozygous unaffected siblings

2007-09-27 | E-MEXP-1076 | biostudies-arrayexpress