Transcription profiling of high quality RNA samples from human breast adenocarcinoma MCF-7 cells and human skeletal muscle to evalutate the experimental performance of Actichip microarrays

ABSTRACT: To evaluate the experimental performance of Actichip microarrays, a series of 10 repeated hybridisation experiments, including 5 dye swaps, were carried out with optimised target labelling, hybridisation, scanning and data analysis protocols. All the procedures were standardised to limit the impact of experimental bias or biological variations on data. The same series of high quality RNA samples purified from human breast adenocarcinoma MCF-7 cells and from human skeletal muscle was used in our experiments. Epithelial cells and muscle tissue were chosen because they express well-characterised sets of cytoskeleton genes, and were anticipated to give well-contrasted differential expression data when analysed with Actichip.

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Homo sapiens

DISEASE(S): breast adenocarcinoma

SUBMITTER: VALLAR Laurent 

PROVIDER: E-MEXP-965 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress