Transcription profiling of pig postischaemic restituted intestinal mucosa after further ischaemic insult

ABSTRACT: Background: Restitution of the surface epithelium is a critical healing process early after ischaemic injury of the gut mucosa. Repeated episodes of intestinal ischaemia occur in many critically ill patients. This study evaluates the resistance of postischaemic restituted intestinal mucosa to a further ischaemic insult. Methods: The superior mesenteric artery (SMA) in pigs was cross-clamped for 1 hour, reperfused for 4 hours, cross-clamped once again for 1 hour, and finally reperfused for 3 hours. Intestinal injury was evaluated with morphometry. Intestinal permeability (from blood to lumen was assessed by transport of fluorescein isothiocyanate dextran (FD-4). Microarray and QRT-PCR analysis were performed.

INSTRUMENT(S): ScanArray Express HT [PerkinElmer]

ORGANISM(S): Sus scrofa

SUBMITTER: Vidar Beisvag 

PROVIDER: E-MEXP-978 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress