Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of heat stress response in Arabidopsis wild type and hsf1x3 double knockout mutant lines

ABSTRACT: The aim of the expression profiling was to define and to apply criteria that allow discrimination between the effects of heat stress (HS) and HSF-dependent gene expression in Arabidopsis. Therefore we determined and analyzed the expression profiles of wild type (WT) and homozygous hsf1--/hsf3-- double knock out mutant plants (abbreviated hsf1/3). Hsf1/3 plants are deficient in HSF1 and HSF3 activity and unable to induce the fast transient expression of mRNAs of a number of HS genes tested (Lohmann et al., 2004).
The effects of HS on gene expression were analyzed after one hour heat treatment (37C) of leaf tissue.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Wolfgang Busch 

PROVIDER: E-MEXP-98 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of novel heat shock factor-dependent genes and biochemical pathways in Arabidopsis thaliana.

Busch Wolfgang W   Wunderlich Markus M   Schöffl Fritz F  

The Plant journal : for cell and molecular biology 20050101 1

In order to assess specific functional roles of plant heat shock transcription factors (HSF) we conducted a transcriptome analysis of Arabidopsis thaliana hsfA1a/hsfA1b double knock out mutants and wild-type plants. We used Affymetrix ATH1 microarrays (representing more than 24 000 genes) and conducted hybridizations for heat-treated or non-heat-treated leaf material of the respective lines. Heat stress had a severe impact on the transcriptome of mutant and wild-type plants. Approximately 11% of  ...[more]