Dataset Information
Transcription profiling of mouse adipocytes to determine the role of RIP140 in adipocyte differentiation
Ontology highlight
ABSTRACT:
Description: RIPKO cells are mouse embryo fibroblasts (MEF) that were derived from
RIP140-knockout mice and have been established as a RIP140 null cell line,
while RIPKO-L7 and RIPKO-L16 are RIPKO cells for which the expression of
RIP140 has been reintroduced using a lentiviral expression system.
Using an established standard cocktail of hormones that includes IBMX,
insulin, dexamethasone and a PPAR gamma agonist the cells were
differentiated into adipocytes and RNA isolated at day 0 and day 10
after the addition of the cocktail.
ORGANISM(S): Mus musculus
SUBMITTER: Malcolm Parker
PROVIDER: E-MIMR-42 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
RIP140-targeted repression of gene expression in adipocytes.
Molecular and cellular biology 20051101 21
Ligand-dependent repression of nuclear receptor activity forms a novel mechanism for regulating gene expression. To investigate the intrinsic role of the corepressor RIP140, we have monitored gene expression profiles in cells that express or lack the RIP140 gene and that can be induced to undergo adipogenesis in vitro. In contrast to normal white adipose tissue and in vitro-differentiated wild-type adipocytes, RIP140-null cells show elevated energy expenditure and express high levels of the unco ...[more]
