Transcriptome profiling of liver and white adipose tissue of male SD rats and CRISPR/Cas-9-mediated heterozygous knockout SD-Nme7+/- male rats

ABSTRACT: We aimed to compare the transcriptome of liver and white adipose tissue of adult male SD rats and the novel CRISPR/Cas-9-mediated heterozygous knockout SD-Nme7+/- adult male rats using Affymetrix Rat Gene 2.1 ST Array Strip. Total RNA was isolated from the liver and visceral adipose tissue (RNeasy Mini Kit, Qiagen) of SD and SDNme7+/- rats. The quality and integrity of the total RNA was evaluated on the Agilent 2100 Bioanalyzer system (Agilent, Palo Alto, CA). Microarray experiments were performed using the Rat Gene 2.1 ST Array Strip. The whole hybridization procedure was performed using the Affymetrix GeneAtlas® system according to the manufacturer's instructions. The quality control of the chips was performed using Affymetrix Expression Console. After applying quality filters and data n

ORGANISM(S): Rattus norvegicus

SUBMITTER: Ondřej Seda 

PROVIDER: E-MTAB-10011 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress