RNase R is associated in a functional complex with the RhpA DEAD-box RNA helicase in Helicobacter pylori

ABSTRACT: Ribonucleases (RNases) are central actors in post-transcriptional regulation, a major level of regulation of gene expression in all cells. This control plays an important role in the bacterial pathogen Helicobacter pylori, although only the function of RNase J was characterized so far. Here, we studied the RNase R enzyme from H. pylori, a 3’-5’ exoribonuclease whose ortholog in Escherichia coli was reported to display, in addition, helicase activity and to be able to hydrolyze RNA substrates with double stranded structures. We observed that HpRNase R protein does not carry the domains responsible for helicase activity in E. coli and accordingly that the purified protein is unable to degrade in vitro RNA molecules with secondary structures. The RNase R helicase domain loss is widespread amo

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Helicobacter pylori

SUBMITTER: Etienne Kornobis 

PROVIDER: E-MTAB-10045 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress