Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

12 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tracking Ebolavirus genomic drift with a resequencing microarray, verification with NGS

ABSTRACT: This study evaluates a resequencing microarray designed to determine the sequence of the four major genes in the Ebolavirus genome, Nucleoprotein (NP), matrix protein (VP40), glycoprotein (GP) and polymerase (L). The array has the ability to determine the sequence of five species and strains: Zaire Ebolavirus (Mayinga and Makona), Bundibugyo Ebolavirus, Sudan Ebolavirus and Tai Forest Ebolavirus. Illumina Next Generation Sequencing verified the sequence of the Zaire Ebolavirus (Mayinga) sample.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Zaire ebolavirus

SUBMITTER: Robert Duncan

PROVIDER: E-MTAB-10102 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Tracking Ebolavirus genomic drift in a replicating virus with a resequencing microarray, verification with NGS 2

Project description:This study demonstrates the ability of an Ebolavirus resequencing microarray to determine the sequence of the Zaire ebolavirus glycoprotein that has been engineered into the place of the surface protein of a recombinant vesicular stomatitis virus expressing green fluorescent protein (rVSV-EBOVgp-GFP). The rVSV-EBOVgp-GFP was cultured in VERO-E6 cells for three passages either in the presence of a monoclonal antibody that blocks infection (KZ52) or in control cultures with no antibody. Culture supernatant and cell lysate was collected before passaging and after each passage. RNA was extracted from each sample and the sequence of the Ebola glycoprotein in each sample was determined by the Ebola resequencing microarray. Illumina Next Generation Sequencing was performed on the initial virus stock, before passaging and on the third passage of the KZ52 antibody selected virus stock to validate the microarray sequence results.

2021-07-02 | E-MTAB-10157 | biostudies-arrayexpress

Tracking Ebolavirus genomic drift with a resequencing microarray

Project description:This study evaluates a resequencing microarray designed to determine the sequence of the four major genes in the Ebolavirus genome, Nucleoprotein (NP), matrix protein (VP40), glycoprotein (GP) and polymerase (L). The array has the ability to determine the sequence of five species and strains: Zaire Ebolavirus (Mayinga and Makona), Bundibugyo Ebolavirus, Sudan Ebolavirus and Tai Forest Ebolavirus.

2021-01-29 | E-MTAB-10007 | biostudies-arrayexpress

Zaire ebolavirus

Project description:Zaire ebolavirus Raw sequence reads

| PRJNA393748 | ENA

Zaire ebolavirus

Project description:Zaire ebolavirus Raw sequence reads

| PRJNA284630 | ENA

Zaire ebolavirus

Project description:Zaire ebolavirus Raw sequence reads

| PRJNA309162 | ENA

Zaire ebolavirus

Project description:Zaire ebolavirus Raw sequence reads

| PRJNA353512 | ENA

Tracking Ebolavirus genomic drift in a replicating virus with a resequencing microarray

Project description:This study demonstrates the ability of a Ebolavirus resequencing microarray to determine the sequence of the Zaire ebolavirus glycoprotein that has been engineered into the place of the surface protein of a recombinant vesicular stomatitis virus expressing green fluorescent protein (rVSV-EBOVgp-GFP). The rVSV-EBOVgp-GFP was cultured in VERO-E6 cells for three passages either in the presence of a monoclonal antibody that blocks infection (KZ52) or in control cultures with no antibody. Culture supernatant and cell lysate was collected before passaging and after each passage. RNA was extracted from each sample and the sequence of the Ebola glycoprotein in each sample was determined by the Ebola resequencing microarray.

2021-01-30 | E-MTAB-10008 | biostudies-arrayexpress

Changes in gene expression in porcine lungs following infection with Zaire ebolavirus

Project description:To elucidate the mechanisms involved in pulmonary inflammation in Zaire ebolavirus-infected pigs, lung sections infected pigs were used for a porcine microarray to analyse changes in gene expression with particular interest in proinflammatory cytokine and chemokine transcription. Pigs were given inhalation anaesthesia and then inoculated with 106 plaque forming units of Zaire ebolavirus in sterile PBS per pig via the intranasal, intraocular and oral routes. Three control pigs were mock inoculated with PBS and housed separately.

2013-05-31 | E-GEOD-44565 | biostudies-arrayexpress

Zaire ebolavirus

Project description:Zaire ebolavirus Genome sequencing

| PRJNA306266 | ENA

Zaire ebolavirus

Project description:Zaire ebolavirus amplicon sequencing

| PRJNA298842 | ENA