RNA-seq differential expression analysis of tmRNA (ssrA) mutants in the genome-reduced bacterium Mycoplasma pneumoniae

ABSTRACT: We used a combination of genetic and proteomic approaches to characterize tmRNA (ssrA) activity in the genome-reduced bacterium Mycoplasma pneumoniae. For this, we generated tmRNA mutants encoding a tag resistant to proteolysis. Endogenous protein tagging by the mutant tmRNA gene (ssrAmk) was then examined by immunoprecipitation (IP) enrichment followed by LC-MS/MS analysis. Additionally, RNA-seq differential expression analysis of the mutants compared to the wild-type strain was assessed.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mycoplasma pneumoniae

SUBMITTER: Luis Serrano 

PROVIDER: E-MTAB-10111 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress