Age associated differences in microbiome in Rhesus Macaque

ABSTRACT: Aging is associated with declining immunity and inflammation as well as alterations in the gut microbiome with a decrease of beneficial microbes and increase in pathogenic ones. The aim of this study was to investigate aging associated gut microbiome in relation to immunologic and metabolic profile in a non-human primate (NHP) model. 12 old (age>18 years) and 4 young (age 3-6 years) Rhesus macaques were included in this study. Immune cell subsets were characterized in PBMC by flow cytometry and plasma cytokines levels were determined by bead based multiplex cytokine analysis. Stool samples were collected by ileal loop and investigated for microbiome analysis by shotgun metagenomics. Serum, gut microbial lysate and microbe-free fecal extract were subjected to metabolomic analysis by mass-sp

INSTRUMENT(S): NextSeq 500

ORGANISM(S): gut metagenome

SUBMITTER: Suresh Pallikkuth 

PROVIDER: E-MTAB-10160 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress