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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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3’RNA-seq of murine CD142− ASPCs co-cultured with CD142+ ASPCs (Aregs) carrying specific gene knockdowns

ABSTRACT: To study the involvement of key Areg (CD142+ ASPC)-specifics factors in the inhibitory capacity of CD142+ ASPCs on adipogenesis, we performed transcriptomic profiling of CD142− ASPCs exposed to the secretome of CD142+ ASPCs carrying knockdowns of the indicated genes. CD142− ASPCs were co-cultured and co-differentiated (within the transwell set-up) with CD142+ ASPCs in which siRNA-based knockdowns of specific genes were performed. ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+ CD34+ SCA1+ cells of the mouse subcutaneous stromal vascular fraction using FACS.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Pernille Rainer

PROVIDER: E-MTAB-10183 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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