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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Circular chromatin conformation capture (4C-seq) experiments for viewpoints on the promoters of Rpgrip1l/Fto, Irx3, Irx5 and Irx6 in mouse hypothalamus

ABSTRACT: We performed 4C to analyze long-range interactions between the promoters of Rpgrip1l/Fto, Irx3, Irx5 and Irx6 and a region in the first intron of the FTO gene that has been associated with obesity. 3 whole mouse hypothalami were used to obtain the data.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Noboru Sakabe

PROVIDER: E-MTAB-10195 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Extensive pleiotropism and allelic heterogeneity mediate metabolic effects of IRX3 and IRX5.

Sobreira Débora R DR Joslin Amelia C AC Zhang Qi Q Williamson Iain I Hansen Grace T GT Farris Kathryn M KM Sakabe Noboru J NJ Sinnott-Armstrong Nasa N Bozek Grazyna G Jensen-Cody Sharon O SO Flippo Kyle H KH Ober Carole C Bickmore Wendy A WA Potthoff Matthew M Chen Mengjie M Claussnitzer Melina M Aneas Ivy I Nóbrega Marcelo A MA

Science (New York, N.Y.) 20210601 6546

Whereas coding variants often have pleiotropic effects across multiple tissues, noncoding variants are thought to mediate their phenotypic effects by specific tissue and temporal regulation of gene expression. Here, we investigated the genetic and functional architecture of a genomic region within the FTO gene that is strongly associated with obesity risk. We show that multiple variants on a common haplotype modify the regulatory properties of several enhancers targeting IRX3 and < ...[more]

PMID: 34083488

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