Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Compressed and non-compressed tissue-engineered HS-deficient and WT cartilage

ABSTRACT: Murine primary rib-cage chondrocytes (mRCs) were isolated from E15.5 WT and HS-deficient Ext1gt/gt mice and expanded for 6-7 days. Tissue-engineered cartilage was generated by a 14-day chondrogenic culture of mRCs at 5×10^5 cells per 25 µl agarose carrier. Engineered cartilage was exposed to cyclic unconfined compression for 3 hours: 10-minute cycles of dynamic unconfined compression (25 % dynamic compression at 1 Hz superimposed to 10 % static off-set) were intermitted by 10-minute breaks (10 % static off-set).

ORGANISM(S): Mus musculus

SUBMITTER: Matthias Gerstner

PROVIDER: E-MTAB-10258 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Successfully replacing damaged cartilage with tissue-engineered constructs requires integration with the host tissue and could benefit from leveraging the native tissue's intrinsic healing capacity; however, efforts are limited by a poor understanding of how cartilage repairs minor defects. Here, we investigated the conditions that foster natural cartilage tissue repair to identify strategies that might be exploited to enhance the integration of engineered/ grafted cartilage with host tissue. We damaged porcine articular cartilage explants and using a combination of pulsed SILAC-based proteomics, ultrastructural imaging, and catabolic enzyme blocking strategies reveal that integration of damaged cartilage surfaces is not driven by neo-matrix synthesis, but rather local depletion of proteoglycans. ADAMTS4 expression and activity are upregulated in injured cartilage explants, but integration could be reduced by inhibiting metalloproteinase activity with TIMP3. These observations suggest that catabolic enzyme-mediated proteoglycan depletion likely allows existing collagen fibrils to undergo cross-linking, fibrillogenesis, or entanglement, driving integration. Catabolic enzymes are often considered pathophysiological markers of osteoarthritis. Our findings suggest that damage-induced upregulation of metalloproteinase activity may be a part of a healing response that tips towards tissue destruction under pathological conditions and in osteoarthritis, but could also be harnessed in tissue engineering strategies to mediate repair. Statement of significance: Cartilage tissue engineering strategies require graft integration with the surrounding tissue; however, how the native tissue repairs minor injuries is poorly understood. We applied pulsed SILACbased proteomics, ultrastructural imaging, and catabolic enzyme blocking strategies to a porcine cartilage explant model and found that integration of damaged cartilage surfaces is driven by catabolic enzyme-mediated local depletion of proteoglycans. Although catabolic enzymes have been implicated in cartilage destruction in osteoarthritis, our findings suggest that damage-induced upregulation of metalloproteinase activity may be a part of a healing response that tips towards tissue destruction under pathological conditions. They also suggest that this natural cartilage tissue repair process could be harnessed in tissue engineering strategies to enhance the integration of engineered cartilage with host tissue.

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Compressed and non-compressed tissue-engineered HS-deficient and WT cartilage

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