Whole genome sequencing analysis of evolved E.coli

ABSTRACT: We created a mutator protein. The mutator, was prepared by fusing a PmCDA1 (Petromyzon marinus Cytidine DeAminase) and E.coli RNA polymerase alpha subunit(EcoRNAP alpha). After 120 cycles, whole genome sequencing was performed on the wild type and evolved sample. After characterization of the mutation capacity of our mutator, we evolved a sucrose utilization strain and we sequenced Suc strain.

INSTRUMENT(S): Illumina NovaSeq 6000, of TruSeq DNA Nano Sample Preparation Kit(Illumina), BIONEER gDNA extraction kit

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Seokhee Kim 

PROVIDER: E-MTAB-10368 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress