A gene regulatory network for neural induction

ABSTRACT: During early vertebrate development, signals from a special region of the embryo, the organizer, can re-direct the fate of non-neural ectoderm cells to form a complete, patterned nervous system. This is called neural induction and has generally been imagined as a single signaling event, causing a switch of fate. Here we undertake a comprehensive analysis, in very fine time-course, of the events following exposure of ectoderm to the organizer. Using transcriptomics and epigenomics we generate a Gene Regulatory Network comprising 175 transcriptional regulators and 5,614 predicted interactions between them, with fine temporal dynamics from initial exposure to the signals to expression of mature neural plate markers. Using in situ hybridization, single-cell RNA-sequencing and reporter assays w

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Gallus gallus

SUBMITTER:  

PROVIDER: E-MTAB-10426 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): E-MTAB-10420

REPOSITORIES: biostudies-arrayexpress