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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of congenic rat strains

ABSTRACT: Expression analysis was carried out in Goto-Kakizaki (GK), Brown Norway (BN) and 24 derived congenic strains using the Illumina RatRef-12 microarray. Each congenic line contains an introgression of a single fragment of the GK strain in the background of the BN recipient strain or vice versa. The resulting lines were fixed by imbreeding. The sizes of the introgressed fragments vary from almost a complete chromosome (consomic) to smaller fragments and some of the fragments overlap. To investigate genetic control of gene expression by the introgressed fragments, we performed transcriptional profiling of liver, soleus muscle and white adipose tissue in 6 months old rats.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Georg Otto

PROVIDER: E-MTAB-1048 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic control of differential acetylation in diabetic rats.

Kaisaki Pamela J PJ Otto Georg W GW McGouran Joanna F JF Toubal Amine A Argoud Karène K Waller-Evans Helen H Finlay Clare C Caldérari Sophie S Bihoreau Marie-Thérèse MT Kessler Benedikt M BM Gauguier Dominique D Mott Richard R

PloS one 20140417 4

Post-translational protein modifications such as acetylation have significant regulatory roles in metabolic processes, but their relationship to both variation in gene expression and DNA sequence is unclear. We address this question in the Goto-Kakizaki (GK) rat inbred strain, a model of polygenic type 2 diabetes. Expression of the NAD-dependent deacetylase Sirtuin-3 is down-regulated in GK rats compared to normoglycemic Brown Norway (BN) rats. We show first that a promoter SNP causes down-regul ...[more]

PMID: 24743600

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