Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single-cell transcriptional landscape of human embryonic limb development - Mouse

ABSTRACT: To investigate into the evolutionary conversation of the single-cell transcriptome of human fetal limbs, especially the principles of forelimb and hindlimb specification and proximal-distal axis establishment, we collected the forelimbs and hindlimbs of mouse embryos matching human samples. We dissected the limbs to separate proximal, middle and distal parts and generated single-cell RNA-seq data of more than 70,000 cells. Combining this dataset with our human data, we are able to see highly conserved limb cell types and limb axis drivers.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Sarah Teichmann 

PROVIDER: E-MTAB-10514 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Human limbs emerge during the fourth post-conception week as mesenchymal buds, which develop into fully formed limbs over the subsequent months<sup>1</sup>. This process is orchestrated by numerous temporally and spatially restricted gene expression programmes, making congenital alterations in phenotype common<sup>2</sup>. Decades of work with model organisms have defined the fundamental mechanisms underlying vertebrate limb development, but an in-depth characterization of this process in humans  ...[more]

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