Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

19 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of hepatic response to anti-diabetic drug and dietary lifestyle interventions in LDLR-/- mice

ABSTRACT: Lean male mice were fed a high fat diet (HFD, lard 24% w/w) for 16 weeks. At 9 weeks, when all hallmarks of prediabetes were established, groups of mice were treated with drug (metformin, glibenclamide, sitagliptin, rosiglitazone, pioglitazone, fenofibrate, T0901317, atorvastatin, salicylate or rofecoxib) for another 7 weeks together with the high fat diet. An additional group was switched back to a chow diet (dietary lifestyle intervention) after the first 9 weeks of high fat diet. All groups were compared to a control group receiving HFD alone and to a reference group fed chow (baseline reference) for the entire experimental period (16 weeks).

ORGANISM(S): Mus musculus

SUBMITTER: Varshna Goelela

PROVIDER: E-MTAB-1063 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling by array of adipose samples from LDLR-/- mice to study response to anti-diabetic drug and dietary lifestyle interventions

Project description:Lean male mice were fed a high fat diet (HFD, lard 24% w/w) for 16 weeks. At 9 weeks, when all hallmarks of prediabetes were established, groups of mice were treated with drug (rosiglitazone, pioglitazone, T0901317, or salicylate) for another 7 weeks together with the high fat diet. An additional group was switched back to a chow diet (dietary lifestyle intervention) after the first 9 weeks of high fat diet. All groups were compared to a control group receiving HFD alone and to a reference group fed chow (baseline reference) for the entire experimental period (16 weeks). One group (n=9) remained on maintenance chow throughout the entire study period (16 weeks) and served as healthy, age-matched control. After the nine week run-in period, the HFD fed mice were matched into thirteen groups based on body weight. The first group (n=9) was sacrificed immediately after matching. The second group (n=15) was continued on HFD until the end of the experiment at t=16 weeks. The fourth group (n=9) was switched to regular chow (dietary lifestyle intervention). The other groups (each n=9) continued on HFD supplemented with drugs typically used in clinical practice. More specifically, following drugs were mixed into HFD ; rosiglitazone (0.010% w/w), pioglitazone (0.010% w/w), T0901317 (0.010% w/w) and salicylate (0.40% w/w).

2014-05-14 | E-GEOD-57659 | biostudies-arrayexpress

Transcription profiling of mouse white adipose tissue from ApoE3L strain exposed to high-fat diet.

Project description:Our aim was to investigate gene expression changes over time in white adipose tissue of ApOE3*Leiden mice on a high-fat diet

2011-01-11 | E-TABM-884 | biostudies-arrayexpress

Transcription profiling of mouse liver response to a glucose challenge in healthy and insulin resistant C57BL/6J mice

Project description:The goal of this experiment is to compare the transcriptional response to a metabolic stressor (glucose bolus) between healthy and insulin resistant mouse liver. Mice with an isogenic background (C57BL/6J mice) were fed diets for 12 weeks containing either 10 or 45% fat by energy (mainly palm oil) after 2 weeks of adaptation to laboratory conditions with a control diet. The animals were subjected to a glucose tolerance test at the end of the feeding trial and liver samples were taken 0 min, 40 min, 2, 18 and 48 h after the glucose challenge. Glucose was injected via the i.p. route at 1 p.m. in all groups and animals had free access to feed during the challenge (no fasting). For more details, see Baccini et al (pmid=19034556)

2012-03-21 | E-MTAB-601 | biostudies-arrayexpress

Transcription profiling by array of mouse peripheral blood mononuclear cells from ApoE3*Leiden mice on a high-fat diet

Project description:The aim of experiment was to investigate gene expression changes over time in liver of ApoE3*Leiden mice on a high-fat diet. Transformed file with remapped and annotated probesets into Entrez gene-ids using the custom MBNI CDF-file (http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/CDF_download_v9.asp, cdf name "Mm430A2_Mm_ENTREZG") is provided as an additional file on the FTP site for this experiment.

2011-04-01 | E-TABM-959 | biostudies-arrayexpress

Mouse F2 Cross, Carbohydrate Diet and High Fat Diet, Heart Tissue

Project description:The effect of high fat diet feeding on heart gene transcription regulation was investigated in an F2 cross of 129S6 x Balb/c mice using Illumina gene expression arrays. Expression data was determined in 5 months old male mice fed a high fat diet (40% fat) for 15 weeks. Control mice were fed a standard carbohydrate chow. 96 animals per group were used. The files mouse-f2-chd-genotype.txt and HFD.txt contain genotyping data linked to the diets, they can be found found in the file E-MTAB-401.additional.zip on the FTP for this submission. NOTE: the file CHD.txt was replaced by the updated file mouse-f2-chd-genotype.txt on 8th April 2014.

2011-09-17 | E-MTAB-401 | biostudies-arrayexpress

Fat BodFat Body, Hungry Brain: Nutriproteomics in the Hypothalamus from a Mouse Model with Obesity and Anorexiay, Hungry Brain: Nutriproteomics in the Hypothalamus from a Mouse Model with Obesity and Anorexia

Project description:Systemic acute inflammatory signals can cause profound anorexia by disrupting the physiological appetite regulation in the hypothalamic milieu. Conversely, obesity related chronic inflammation of the hypothalamus can disturb anorexigenic signals and promote abnormal body weight control. The aim of the present study was to compare the global hypothalamic endophenotype in C57/Bl6 mice exposed to a high-fat diet or with acute illness mediated by LPS. Ten-week old male C57/Bl6 mice (n=18) were randomly divided into four groups; the control 1 group (n =3) was fed a normal diet whereas the high-fat diet (HFD) group (n =6) was fed a high-fat diet for eight weeks. The control 2 group (n=3) received an intraperitoneal injection of saline whereas the LPS group (n=6) received an intraperitoneal injection of LPS. Mice were sacrificed 18-hr post-injection. Both control 2 and LPS groups were fed a normal diet for eight weeks before the injection. The hypothalamic regions were removed and analysed using a 2D LC-MS methodology. The proteomic analysis profiled 9,235 proteins (q<0.05) across all biological states, of which 522 proteins were found modulated in the HFD group and another 579 in the LPS group. The proteomic profiles demonstrated that the systemic acute inflammation linked with anorexia induced a negative feedback loop of appetite control in the hypothalamus, suggesting an effort to re-establish homeostasis. By contrast, the chronic inflammation associated with obesity initiated a “perpetual cycle” of positive feedback enhancement of appetite regulation further exacerbating positive energy balance.

2018-01-29 | PXD002119 | Pride

Transcription profiling of liver, skeletal muscle and white adipose tissue from ApoE3L mice

Project description:Our aim was to investigate gene expression changes over time in liver of ApOE3*Leiden mice on a high-fat diet. Probesets were remapped and annotated into Entrez gene-ids using the custom MBNI CDF-file, version 9.0.1 - the GCRMA data generated

2010-07-28 | E-TABM-1039 | biostudies-arrayexpress

Red-fleshed apples rich in anthocyanins and white-fleshed apples poor in anthocyanins modulate aorta and heart proteome in hypercholesterolemic rats. The AppleCOR study

Project description:New mechanisms-of-action of anthocyanins (ACNs) provided by a red-fleshed apple compared with a white-fleshed apple ACN-poor, and with an ACN-rich extract on the proteome profile of aorta and heart as cardiovascular key tissues were determined. Hypercholesterolemic Wistar rats were separated into the corresponding groups to analyze the proteomic profile of the aorta and heart tissues using nano-liquid chromatography coupled to mass-spectrometry. Red-fleshed apple downregulated CRP, C1QB and CFP related-inflammation. White-fleshed apple reduced C1QB, CFB, CFD, C3, and C9 related to the complement system, reduced MB and CP related to iron metabolism, and increased ME1, PKM, and PC related to energy homeostasis. ACN-rich extract increased FMOD, TAGLN, and CAP1 related to cellular structure and decreased PRKACA, IQGAP1, and HSP90AB1 related to cellular signaling. Red-fleshed apple rich in ACNs suggested an anti-inflammatory effect while white-fleshed apple reduced the complement system protein-related. An apple matrix effect reduced inflammatory proteins regardless their ACN content.

2022-04-04 | PXD018885 | Pride

Gene expression in the liver, effect of maternal high-fat diet during or prior to pregnancy

Project description:The present study aimed to examine the effect of high-fat diet prior to pregnancy on the liver of mouse offspring. Female C57BL/6J mice were fed a normal chow (15.2% fat by energy) (CTR and CTR-PP groups) or a high-fat chow (31.2% fat by energy) (HFD and HFD-PP groups) for 3−4 weeks and then mated with male C57BL/6J mice fed normal chow. Some mothers continued on the same diet until pups reached 21 days of age (CTR and HFD), and others were fed the different chows from gestational day 0 (CTR-PP and HFD-PP) to determine the effects of a high-fat diet during the pre-pregnancy period in HFD-PP/CTR and HFD/CTR-PP comparisons. RNA sample was taken from liver of 3-week-old mouse prenatally received high-fat diet prior to pregnancy, during pregnancy and lactation, or through prior to and during pregnancy and lactation, while control RNA was taken from control counterpart prenatally received normal diet alone. Comparisons among groups were made by one-color method with normalized data from Cy3 channels for data analysis.

2013-06-18 | E-GEOD-48014 | biostudies-arrayexpress

Computational Prediction, Validation and Repurposing of Existing Drug as Inhibitor of Vascular Calcification

Project description:This study aimed to identify small molecules that inhibit vascular calcification with computational approach. To examine the mechanism of calcification inhibition, proteomics analysis was performed using aorta tissue samples to clarify therapeutic target.

2022-02-17 | PXD029963 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data