Nitric oxide exposure induces production of Rpf-dependent differentially culturable mycobacteria

ABSTRACT: Rationale: Tuberculosis has a devastating impact on global health by claiming nearly 1.4 million lives each year. During infection Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, produces heterogenous populations some of which don’t produce colonies on agar but grow in liquid media and often require supplementation with culture supernatants or recombinant Resuscitation-promoting factor, thus defined as differentially culturable bacilli. Objectives: to evaluate whether exposure to nitric oxide (NO), a well-known host defence molecule, alters mycobacterial growth phenotypes and drives generation of Rpf-dependent differentially culturable bacilli. Methods: a novel NO donor was synthesised and tested against Mtb and Mycobacterium bovis BCG in vitro, followed by growth

ORGANISM(S): Mycobacterium tuberculosis H37Rv

SUBMITTER: Simon Waddell 

PROVIDER: E-MTAB-10776 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress