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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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DRIP-seq files for: DDX17 is required for efficient DSB repair at DNA:RNA hybrid deficient loci

ABSTRACT: Determining the role of DDX17 in the formation of DNA:RNA-hybrids around active DNA double-strand breaks (DSBs) using DRIP-seq in the damaged induced via AsiSI (DIvA) cell system that induced DSBs at known genomic loci in response to hydroxytamoxifen (OHT) treatment via and AsiSI enzyme fused to an oestrogen receptor. Sequencing was done using either control or DDX17 siRNA, and mock or 4 hours 300nM OHT treatment. Paired-end 150 cycles was completed on an Illumina NextSeq 500 and library prep was completed using the NEB NEBNext Ultra II library prep kit.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Aldo Bader

PROVIDER: E-MTAB-10814 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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