IiCLIP protocol benchmarking with PTBP1

ABSTRACT: We describe an improved individual nucleotide resolution CLIP protocol (iiCLIP), which can be completed within 4 days from UV crosslinking to libraries for sequencing. For benchmarking, we directly compared PTBP1 iiCLIP libraries with the iCLIP2 protocol produced under standardised conditions with 1 million HEK293 cells, and with public eCLIP and iCLIP PTBP1 data. There are 3 PTBP1 iiCLIP libraries, 1 input iiCLIP library and 1 PTBP1 iCLIP2 library produced in this study.

INSTRUMENT(S): Illumina MiSeq, Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Flora Lee 

PROVIDER: E-MTAB-10881 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress