Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of peripheral blood mononuclear cells taken from rheumatoid arthritis patients before and after treatment with Ethanercept (Enbrel)

ABSTRACT: Rheumatoid Arthritis patients were with Ethanercept (Enbrel), blocking the TNF alpha signalcascade by receptor mimicri. Peripheral blood samples were taken at 3 timepoints, before the first and 2 next drug injections after 3 and 6 days (t0, t1 (72h), t2 (144h)). Peripheral blood mononuclear cells were isolated by standard Ficoll gradient centrifugation. Whole RNA samples were profiled on Affymetrix Human Genome U95A and Human Genome U133A arrays. Later time point were analysed against timepoint 0 and differential gene expression signatures were correlated to clinical data i.e. responsiveness.

This experiment was reloaded into ArrayExpress on 27th January 2010 to fix errors in the CEL and CHP file association with hybridizations.

ORGANISM(S): Homo sapiens

SUBMITTER: Dirk Koczan 

PROVIDER: E-MTAB-11 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Molecular discrimination of responders and nonresponders to anti-TNF alpha therapy in rheumatoid arthritis by etanercept.

Koczan Dirk D   Drynda Susanne S   Hecker Michael M   Drynda Andreas A   Guthke Reinhard R   Kekow Joern J   Thiesen Hans-Juergen HJ  

Arthritis research & therapy 20080502 3

<h4>Introduction</h4>About 30% of rheumatoid arthritis patients fail to respond adequately to TNFalpha-blocking therapy. There is a medical and socioeconomic need to identify molecular markers for an early prediction of responders and nonresponders.<h4>Methods</h4>RNA was extracted from peripheral blood mononuclear cells of 19 rheumatoid arthritis patients before the first application of the TNFalpha blocker etanercept as well as after 72 hours. Clinical response was assessed over 3 months using  ...[more]

Similar Datasets

Transcriptomics Hypoxic tubular endothelium and EPC MVs
2011-01-29 | E-MEXP-2972 | biostudies-arrayexpress
Transcriptomics Transcription profiling of Synechocystis cells exposed to high light levels
2009-04-23 | E-TABM-333 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human invasive breast cancer carcinomas with different histological types and invasive lobular carcinoma
2010-08-20 | E-NCMF-3 | biostudies-arrayexpress
Transcriptomics Transcription profiling by array of mouse treated with Antiotensin II to induce hypertension in either a chronic or acute fashion
2005-04-30 | E-TIGR-121 | biostudies-arrayexpress
Genomics Affymetrix 250K StyI SNP array data from PBMCs and cell lines of metastatic melanoma patients
2010-04-13 | GSE17534 | GEO
Transcriptomics HS-R1
2003-12-16 | GSE669 | GEO
Transcriptomics Transcription profiling by array of human 16 cell lines to investigate correlated and anticorrelated expression
2008-06-11 | E-GEOD-10021 | biostudies-arrayexpress
Transcriptomics Transcription profiling of SBH/y rats vs SBN/y rats under basal conditions and after salt loading
2004-09-10 | E-MEXP-157 | biostudies-arrayexpress
Transcriptomics Transcription profiling by array of human normal pre-frontal cortex, liver, and colon tissues and colon tumors
2009-01-23 | E-GEOD-13471 | biostudies-arrayexpress
Genomics Mus musculus
| PRJNA90199 | ENA